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De Ita JR, Castilla-Cortzar I, Aguirre GA et al (2015) Altered liver expression of genes involved in lipid and glucose metabolism in mice with partial IGF-1 deficiency: an experimental approach to metabolic syndrome

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After centrifugation, the samples were incubated overnight at 4 C with the addition of anti-PGC1 or anti-IgG according to the antibody manufacturers instructions

Intranasal administration, though less studied with Dihexa specifically, would theoretically provide the most direct CNS delivery with minimal systemic exposurepotentially requiring even less frequent dosing to maintain effective brain tissue concentrations