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Am J Med (2007) 120(2):1517

Pal, C., Bengtsson-Palme, J., Rensing, C., Kristiansson, E

He recalled the teenager raising his hand and asking, If you take two beakers with equal volumes of water, one at 35C and the other at 100C, and put them into a refrigerator, the one that started at 100C freezes first

After three weeks of cryopreservation, the frozen straws were removed from the liquid nitrogen and thawed in a 37 C water bath for 30 s, then incubated at 37 C for subsequent use