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After centrifugation at 20,000 g for 1 h, supernatants were incubated with Ni 2+ Sepharose and applied to two successive washes with buffer A (50 mM Tris pH 7.5, 1.2 M KCl, 20 mM imidazole) and buffer B (50 mM Tris pH 7.5, 150 mM NaCl)

Skin thinning can cause sensitivity, redness, dry and oily skin

Seelinger G, Merfort I, Schempp CM

Additionally, the impact of processing on the physiological activities of garlics sulfur-containing compounds is discussed, and future research directions are proposed to provide a theoretical basis for optimizing garlic processing techniques and developing functional products

Ideal for separation and quantification of chemical components in different samples