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Description

The dried residues were dissolved in water, in which 1 10 4 cells/ 5 L were used for the phi29 polymerase-based RCA reaction mixture

Endocrine connections 13: 2024

The serum-free culture supernatant derived from EBC-1 cells (EBC-1 supernatant) and the cells were spun down, and each supernatant was filtrated through a 0.45-m polyvinylidene difluoride membrane filter (Merck Millipore, Italy) and concentrated by ultrafiltration (Amicon Ultra 3K, Merck Millipore)