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The method, use, or composition according to claim 23, wherein the subject is about 4.0 years of age, 4.1 years of age, 4.2 years of age, 4.3 years of age, 4.4 years of age, or 4.5 years of age

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10.1016/j.cellimm.2021.104364

To improve purification of rMCP, insoluble pellet fractions were resuspended in incubation buffer (20 mM Tris-HCl, pH 7.4, 0.5 M NaCl, 6 M GdmCl) on ice for 20 min

This critical step prevents condensation from forming inside the vial when bacteriostatic water is added, which can: Introduce uncontrolled water into the reconstitution (altering final concentration) Create localized temperature differentials affecting peptide stability Complicate visual verification of complete reconstitution Step 3 Sterilize the Vial Top and Prepare Bacteriostatic Water Using an alcohol prep pad, thoroughly swab the rubber stopper of both the peptide vial and bacteriostatic water vial