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The role of tetrahydrobiopterin in the regulation of neuronal nitric-oxide synthase-generated superoxide

Another critical limitation lies in the complexity of dosing and timing

Mass spectrometry analyses The purified PGRMC1 (a.a.44195) proteins, the wild-type (apo and haem) and the C129S mutant (apo and haem), which included additional amino acid residues (GPLGSEF), were buffer-exchanged into 100 mmol l 1 ammonium acetate, pH 7.5, by passing the proteins through a Bio-Spin 6 column (Bio-Rad)

doi: 10.1093/besa/22.1.31 107 SpanierE