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Although both CYP1A and CYP3A isoforms oxidize AFB 1 , there are conflicting reports on their relative importance [63]

One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

Use it each night then rinse it off after about 30 minutes

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