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The only difference is how your protein of interest is connected to the agarose beads, which has important implications for how you elute it, and any proteins that it binds to from the column

Furthermore, to evaluate apoptosis induction, the terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay was performed using the in situ cell death detection kit

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