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In terms of specific regulatory effects, the protective role of mitophagy is mainly reflected in a state of moderate activation: when cells are subjected to mild stress and mitochondria undergo local damage, mitophagy can specifically recognize and clear damaged, depolarized mitochondria, effectively reducing the abnormal release of ROS and the cytoplasmic leakage of mtDNA

Application: Western Blot 1-1000 ng/lane of GFP C-terminally tagged with Strep-tagII (28 kDa) were separated by SDS-PAGE and blotted onto a membrane

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We hope this approach can contribute to the development of more effective mRNA-based prodrugs