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As we explained (88 FR 82099 through 82100), because the estimated allowed amount data element is meant to provide an estimate of what the algorithm produces in dollars, across the universe covered by a particular payer's plan, such an amount should reflect the amount the hospital expects to be reimbursed for the item or service (or service package), on average

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Allow to separate, draw off the lower layer into a second small separator, add 5 mL of 5 N sulfuric acid, shake well, and allow to separate completely (the complete separation of the layer may be facilitated by centrifuging): the separated upper layer is colorless or has no more color than a mixture of 0.15 mL of 0.10 N potassium permanganate and 250 mL of water

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