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Under proposed temporary Rule 510T (which would expire two years after its effective date), an issuer conducting a Rule 506(c) offering would be required to submit to the SEC (no later than the date of first use of such materials in the offering) any written general solicitation materials prepared by or on behalf of the issuer and used in connection with the offering

The resulting chromatogram for the R49Q mutant showed a single peak that mostly paralleled the hexameric WT sample, but also showed a minor dimer peak

Protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) at a constant voltage of 100 V for 1 h

10.1155/2023/5075581 [DOI] [PMC free article] [PubMed] [Google Scholar] Riaz M., Khalid R., Afzal M., Anjum F., Fatima H., Zia S., et al