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Concerns about acetaminophen largely stem from observational studies, which identify patterns but cannot prove cause and effect

Histopathological analyses and immunohistochemistry Animals were sacrificed with carbon dioxide and perfused with PBS followed by 4% formaldehyde and post-fixed in formaldehyde for 24 h followed by 3 d in 40% sucrose, snap frozen in iso-pentane and kept at 80 C

This lively debate underscores the importance of accurate parameters when extrapolating in vitro enzyme data: the two hypotheses are not mutually exclusive, but remind us that we must directly and precisely quantify substrate concentrations in the mitochondrial matrix when constructing physiological models

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