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Benvenga S, Vita R, Di Bari F, Granese R, Metro D, Le Donne M (2019) Stable consumption of swordfish favors, whereas stable consumption of oily fish protects from, development of postpartum thyroiditis

10.1002/jmv.1890150110 40

Figure 1 The upstream and downstream homologous arms of gr were amplified by PCR using primer pairs of gr UF/ gr UR [819 bp upstream homologous arm (UHA)] and gr DF/ gr DR [933 bp downstream homologous arm (DHA)]

The kanamycin cassette was removed from the resulting strains using the pCP20 helper plasmid [19] with the formation of the corresponding deletions ( cysB, cysE, gshA, gshB and emaA )