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Reclast is contraindicated in patients with creatinine clearance less than 35 mL/min [see Contraindications (4)]

A modification of the assay developed for flavoproteins has been reported and was utilized here 51

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For this purpose, RAW 264.7 cells, a macrophage cell line obtained from a male mouse tumor induced with Abelson murine leukemia virus, were plated in complete DMEM (Euroclone) supplemented with 10% FBS in 48-well plates at a density of 2 10 5 cells per well