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Plasma and erythrocyte free amino acid quantification Heparin-anticoagulated WB was centrifuged at 4 (sd 2)C for 30 min at 3000 g (cross-sectional study) and 10 min at 2000 g (GLY inclusion level and supplementation studies) to separate the plasma, buffy coat and erythrocytes

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Moreover, in patients with type 2 diabetes, hyperglycemia promotes the maintenance of pro-inflammatory state [95, 117, 118]

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Supplementation When Needed For individuals at higher risk, such as pregnant women, elderly adults, or strict vegetarians, oral B12 supplements may be advised even before symptoms appear