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Here, GGT knockout mutant was shown to produce an increased amount of medium-sized PGA fragments (1 10 5 Da) as compared to the wild-type strain, in which both the amount and the size of PGA fragments (5 10 3 Da) decreased considerably

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Dec 2016;104(6):1534-1544

Samples were separated on 5-20% gradient polyacrylamide gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories)