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As a result, phosphospecific antibodies are produced (5)

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Figure 10 In addition to the sulfonamides, e.g., 40 and 41 , the spiro-oxindole dihydroquinazolinone derivative 42 was identified in the screening campaign and subsequently a large series of related analogues were made, e.g., by applying rapid MW-assisted reactions and thereafter examined as IRAP inhibitors in bioassays (Engen et al., 2020) (Figure 10)

Peptides work at the cell and tissue level

HBTU hexafluorophosphate N-oxide the peptide resin is incubated with 20% of the appropriate acyl anhydride in DMF and DIPEA (1.5 equivalents) for 30 minutes at room temperature