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Cells were then trypsinized and transferred into clear flow cytometry tubes containing phosphate buffer saline (PBS) supplemented with 2 % FBS

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The resuspended cultures of eYFP-GLYR1 and the peroxisome marker were combined in 1:1 ratio by volume and infiltrated into 6-week-old Nicotiana tabacum leaves with a 1 mL needleless syringe

Many studies only report superficial phenotypic changes, such as Th17/Treg ratios or related cytokines, which greatly reduces the comparability and reproducibility of the research

It focused on equipping residents with the resources needed for confident HCV management, especially in an area disproportionately impacted by the virus