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*Correspondence: Bonglee Kim, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Yes, HCG can influence estradiol (E2) levels

Other notable techniques include plasma-free amino acid dose-response (Whitehouse et al., 2017), in situ and in vitro methods (Estes et al., 2022), and fecal amino acid excretion (Risnen et al., 2020), all of which are employed to evaluate rumen-protected amino acids

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L2-Cmu development and validation To target IGF-1R action in mice, we utilized the mAb, L2-Cmu (Amgen Inc, Thousand Oaks, CA), which is a murinized IgG1 version of the fully human L2-C mAb previously reported by Calzone et al