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Microarray analysis was performed to identify heart-specific transcriptomic changes in BaP/fluoranthene (FL) during early development, with Ca 2+ cycling and muscle contraction genes being the most differentially expressed class of transcripts (Table 2)

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Immunoblotting Cells were washed with ice-cold PBS and lysed with RIPA lysis buffer

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IV delivery allows all of these nutrients to reach peak blood levels simultaneously, supporting the antioxidant recycling chain