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In one study of 127 young mean age, 20.3 years) women, supplementation with a high dose of thiamin (50 mg/day

RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

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Direct detection of bla KPC-2 plasmid of carbapenem resistant isolates in wastewater may be used as an effective epidemiological indicator