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Lysates of Raw264.7 cells were prepared with lysis buffer containing 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 10% glycerol, 150 mM NaCl, 1% NP-40, and protease/phosphatase inhibitors (Thermo Fisher Scientific) and incubated with the peptide-conjugate resins for 1 h at 4C

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