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Protein levels were determined by Western blot assay using the NuPAGE 4 to 12 %, Bis-Tris, 1.0 mm Mini Protein Gel (NP0321) with the WesternBreeze Chemiluminescent Kit (ThermoFisher Scientific, USA) according to the method of Doganlar and colleagues (2021[16])

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Mehra & Maji (2019) show how SEC is being used to study the aggregation behavior of alpha-synuclein protein that is associated with Parkinsons disease

Epithalon and Anti-Mutagenic Effects A 2011 study examined Epithalons effect on chromosomal aberrations in three mouse models: SAMP-1 (senescence-accelerated mice) SAMR-1 (control strain) SHR (spontaneously hypertensive rats, female mice)(8) Key findings: At baseline, SAMP-1 mice showed nearly twice the chromosomal aberration rate of the other strains Epithalon exposure at 2 months of age reduced chromosomal aberrations across all models The most pronounced decrease was seen in the SAMP-1 accelerated-aging mice Co-administration with melatonin did not significantly alter Epithalons observed action These results support a potential anti-mutagenic role of Epithalon in bone marrow cells under specific experimental conditions

Information Processing Systems - Open Systems Interconnection Reference Model - Part 2: Security Architecture