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The body uses carotenoids like beta-carotene, lycopene, and lutein as powerful antioxidants

In a microwell ELISA plate pre-coated with anti-human glutathione peroxidase 4 (GPX4) antibody (solid phase antibody), add human glutathione peroxidase 4 (GPX4) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate

In allergic reactions, immediate hypersensitivity reactions are the most common, but delayed hypersensitivity reactions may also occur

The lack of treatments is in part due to the complexity of the disease, as not only multiple genetic and environmental risk factors but also different cell types within the inner and outer retina, have been shown to be involved in the pathophysiology of AMD (Malek and Lad, 2014

doi: 10.1016/0308-8146(80)90027-8 94