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Animal numbers were chosen with consideration of ethical requirements and the 3Rs principle (replacement, reduction, refinement) to balance statistical sensitivity with minimizing animal use 18

Competition between DNA methylation and transcription factors determines binding of NRF1

And there were no changes observed in the intestinal microbiome when participants adopted a low-FODMAP diet

The cause, according to Aretaeus, was sometimes either another chronic disease or even consuming "a copious draught of cold water." [70] [71] The paediatrician Samuel Gee gave the first modern-day description of the condition in children in a lecture at the Hospital for Sick Children, Great Ormond Street, London, in 1887