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Prepared cDNA was used in the qPCR analyzer (StepOne, Applied Biosystems, Singapore) using MAXIMA SYBR Green qPCR Master Mix with the following program: 1 cycle at 95 C for 10 min

Benza RL, Gomberg-Maitland M, Demarco T, Frost AE, Torbicki A, Langleben D, et al

After magnetic removing the beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)

Also, bile acids metabolism has been described as involved in atherosclerosis development: it is probably related to bacterial bile salt hydrolyzation, which can stimulate enlargement of atherosclerotic plaque through cholesterol accumulation and foam cell formation [68]

London: San Diego Academic Press