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After segmentation, we calculated the fluorescence traces from soma and neurite ROIs, and subtracted from them the fluorescence traces of the surrounding neuropil rings

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Finally, the E2 ligase will cooperatively transfer the ubiquitin chain with a specified E3 ligase to a particular substrate, whereby the 26S proteasome will target the polyubiquitinated protein for degradation ( Figure 1 )

Based on the supporting evidence in mammals, we sought to understand the significance of this putative phosphorylation event in worms