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The convergence or close proximity of some HOMOs with high electron density with those of negative electrostatic potential give further support to the idea that the positions may indeed be subject to electrostatic attack

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The day after the fragments were transferred to Dulbeccos Modified Eagle Medium (DMEM) with high glucose concentration supplemented with 20% FBS, 2 mM L-glutamine, 100 U/mL penicillin, 100 g/mL streptomycin and 1% non-essential amino acids