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Autophagy inhibits cancer stemness in triple-negative breast cancer via miR-181a-mediated regulation of ATG5 and/or ATG2B

Time-lapse microscopy For videos of Sema4D-Fc-647 endocytosis, HeLa cells expressing GFP-tubulin and mCherry-Histone plated in chamber slides were incubated with Sema4D-Fc-Alexa647 in FluoroBrite DMEM Media (Thermo Fisher Scientific) with 10% serum, 1 mM sodium pyruvate and 2 mM glutamine and imaged using a Nikon Inverted spinning disk confocal microscope at 40

Furthermore, before considering SQOR-based therapies, it will be necessary to develop companion diagnostic tools to assess SQOR activity in a patients tissues and determine how dependent cell survival is on SQOR, thereby guiding whether to inhibit or activate SQOR to achieve the desired therapeutic outcome

and C16A/C84A/C130A/C159A, a D1 disulfide and CSD deletant (Cerutti et al., 2014)

Hence, the intracellular redox state is suggested to be maintained by a SKN-1/WDR-23-dependent autoregulatory loop