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In the absence of GPR146, impaired adipocyte differentiation and reduced lipolytic capacity together lead to diminished efflux of free fatty acids (FFAs) from adipose tissue

Consequently, it may ameliorate ischemic stroke in diabetic mice (224)

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To activate the non-canonical inflammasome, peritoneal macrophages were stimulated with LPS (100 ng/mL) for 3 hr followed by intracellular LPS delivery (1 g/mL) by transfection using lipofectamine 2000 (Invitrogen, 11668027), according to the manufacturers protocol, in OptiMEM (Gibco, 31985-070) for 16hr