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Plates were washed with PBS-T and anti-mouse IgM-HRP or anti-mouse IgG-HRP (Sigma A8786 and A3673) was used for detection

With the recent de-orphaning of SLC22A15 as a second ergothioneine transporter expressed highly in the brain (Reference Yee, Buitrago and Stecula28) , and likelihood of further ergothioneine transporters yet to be characterised (see Fig