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HFD impairs liver glutathione biosynthesis, vitamin D metabolism genes and genes associated with non-alcoholic fatty liver disease (NAFLD) Genes involved in the GSH biosynthesis pathway were significantly downregulated in the livers of mice fed an HFD compared to those of mice fed a healthy diet (controls) (Fig

A total of 20 g protein was warmed to 37 C for 30 min with a sample buffer before loading onto 12% SDS-PAGE gels (Thermo Fisher Scientific BR161-0158)