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To further confirm the presence of deaminated glutathione, 10 L solution from the injection vial was mixed with 40 L of derivatization reagent (MeOH: H 2 O, 4:1, v/v, containing 25 mM NEM and 10 mM ammonium formate, pH 7.0) followed by incubation on ice for 30 min and LC-MS analysis