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From this solution AC (17.6 mm), another solution prepared with using of sterile PBS.2 mL of solution AC plus 2 mL PBS used for preparation of solution BC (8.8 mM) and then 2 mL of solution BC plus 2 mL PBS used for making of solution CC (4.4 mM) and finally 2 mL of solution CC plus 2 mL PBS mixed for preparation of solution DC (2.2 mM)

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A Pierce BCA Protein Assay was utilized to determine protein concentration in the membrane, according to manufacturers instructions

Oxidative and endoplasmic reticulum stress interplay in sporadic amyotrophic lateral sclerosis

These cells are then grown in a lab, where they form groups of cells and can change into different types of cells, like cartilage and fat cells