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doi: 10.1016/j.heliyon.2023.e22443 47 LuLOvesonBCJoYJLauerTWUsuiSKomeimaKet al

plasmid #136466, Watertown, MA, United States) plasmids was performed with the transfection reagent FuGENE HD (Promega, Ref: E2311, Promega GmbH, Walldorf, Germany) at a 2:1 FuGENE HD:DNA ratio according to the manufacturers protocol