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LC-MS/MS conditions The analyte molecules were eluted on Zorbax XDB-C18 column (3.5 m, 2.1x20 mm) with the mobile phase composed of water/0.1% formic acid and methanol/0.1% formic acid in the ratio of 70:30 V/V with the flow rate of 0.2 ml/min

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Although the benchmark year assignment data generated during the performance year being reconciled would be based on the same ACO participant list, assignment methodology selection under 425.226(a)(1), and assignment methodology under subpart E of Part 425 of the regulations as used in ( calculating the ACO's most recent prior benchmark, other factors, such as more recent Medicare beneficiary eligibility data along with the ACOs included in the claims-based assignment competition, could differ and impact an ACO's assigned population

In particular, except for inflammatory bowel disease where there is a definite primary intestinal pathology, it is unclear if the disruption in the intestinal barrier function is primary and be responsible for the systemic diseases or secondary to other causes associated with the primary systemic diseases

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