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In light of the observed background of these metabolites in rats, which may also occur in humans, and the fact that our calculations were based on relative excretion rates of the completely N -acetylated metabolites in rats, we consider that analysis of NAcCys-BDA-Lys and NAcCys-BDA-Lys in human urine is unlikely to provide accurate estimates of human exposure to furan

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The application of an isotopically labeled standard of patulin and a matrix-matched methodology minimize these effects and are generally employed when developing analytical methods for patulin quantification