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Maximum tiotropium plasma concentrations were observed 5 to 7 minutes after inhalation

In an in vitro study, Liu et al

Dai Y, Li J, Wang T, Zhang X,Du P

2.6.3 Gas chromatography analysis SCFA concentrations were determined using the gas chromatograph Agilent 8860 (Agilent, USA) equipped with a flame ionization detector and a BPX70 capillary column (60 m x 0.25 mm x 0.25 m

Additionally, signaling pathways such as PI3K/Akt/mTOR and metabolites like succinate can also regulate the expression and stability of HIF-1 (71, 72)