demonstrating glutathione production in neurons and astrocytes experiment Metabolism of the Brain—The Role of Re)building the nervous system: A

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Increased sirtuin activity can help boost metabolism, decrease inflammation, extend cell life, and prevent neurodegeneration

Western blotting Whole cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% of 2-mercaptoethanol (Bio-Rad)

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showed that AS-IV activates the PI3K/AKT/Nrf2/ARE signaling pathway, enhancing Nrf2 expression and nuclear translocation, binding to the ARE upstream, and increasing downstream antioxidant protein expression (e.g., Ho-1, Gpx-1, Gclc, and Gclm), which mitigates oxidative stress in glomerular mesangial cells (GMCs) induced by high glucose