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For reasons currently unknown, we noted that dhs-28 animals grown and stained on 10-cm NGM/OP50/Nile Red plates displayed more prominent 590 nm and 610 nm peaks of Spec-2s than their counterparts that were grown and stained on 6-cm NGM/ OP50/Nile Red plates (compare Additional File 3D with Figure 3G)

PGC-1 and exercise intensity dependent adaptations in mouse skeletal muscle

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Besides, most newbies have a muscular growth spurt when they start lifting because that's when nearly anything works