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Choroidal endothelial cells isolated from PEDF knockout mice demonstrated heightened sensitivity to hydrogen peroxide challenge with an increase in apoptotic cells, oxidative stress, and pro-inflammatory cytokine profile, along with increased cellular proliferation, decreased adhesion and migration (Park et al., 2011)

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The solvent was then evaporated using a rotary evaporator under a vacuum (50 C) for 15 min to form a thin layer of lipids on the walls of the round bottom flask

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