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Beta-catenin signaling, liver regeneration and hepatocellular cancer: sorting the good from the bad

Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific 15-340-154) containing lysis buffer (Tris-HCl 100 mM, 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor Cocktail and homogenized using a bead mill (VWR) for 10 seconds

Minor structural variations or leftover synthesis reagents can completely invalidate assay outcomes

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Thrombin generates previously unidentified C5 products that support the terminal complement activation pathway