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Individual slices were transferred to a submerged chamber that was mounted on the stage of an upright microscope, and perfused with normal aCSF with 1.5 mM MgCl 2 and 2.5 mM CaCl 2 at 31 C

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The AAP also considers spironolactone, a potasium sparing diuretic that may represent a preferable alternative, as usually compatible with breast feeding

Nutrients 11 (7), 1549