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Electrophysiological recordings further supported this, revealing that Dihexa caused a 1.6-fold increase and Nle-AngIV caused a 1.7-fold increase in the frequency of AMPA-mediated mEPSCs compared to vehicle-treated neurons, indicating an expansion of functional synapses without altering individual synapse properties such as amplitude, rise, or decay times

Pre-processing of PPG is required to get a clean waveform pattern (36)

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However, because retatrutide has the additional glucagon receptor component, starting at a lower-than-expected dose and titrating up remains the safest approach