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Flavonoids can be summarized to reverse MDR via multiple mechanisms such as regulating the ABC transporters, boosting ROS production in cancer cells, or conversely inhibiting the activities of the anti-oxidative enzymes, subduing the proliferation of cancer stem cells, inhibiting EMT (epithelialmesenchymal transition) signalling, modulating autophagy and the related factors, and lastly, by distorting the progression of cell cycle in cancer cells [202]

The PCR reaction was carried out at 94 C for 5 min, 35 cycles of 94 C for 30 s, 50 C for 30 s, and 72 C for 30 s, with a final extension of 72 C for 10 min

After fixation, cells were permeabilized with 0.1% Triton X-100 for 15 min, followed by blocking with 1% BSA for 30 min at room temperature