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Experimental Design and Methodology The study used both male and female 5xFAD transgenic mice, aged four months at the onset of testing

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In pulmonary fibrosis studies using bleomycin to induce lung injury in mice, GHK-Cu doses of 0.0026 to 0.26 mg/mL/day administered every other day reduced inflammatory cell infiltration, decreased interstitial thickness (the scarring that stiffens lung tissue), and lowered TNF-alpha and IL-6 expression

In this context, microneedling monotherapy means microneedling without any additional topical medications