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Heres a graphical overview of the synthesis pathways: Glutamate from glutamine: Glutamate can be synthesized from the amino acid glutamine with the help of the enzyme glutaminase (GLS and GLS2 genes)

All the samples were blended with 2% sodium dodecyl sulfate (SDS) and then electrophoresed on agarose gel after incubation for 10 min at room temperature

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