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In an ideal world, preclinical and clinical studies would always use the same techniques (e.g., the same anticoagulant for sample collection, process samples from one individual at a time, control for environmental confounds, etc.)

This work was supported in part by the National Institute of Diabetes and Digestive and Kidney Diseases Grants [R01 DK 070195], [R01 DK102142], and [R01 DK125465], the National Institute of General Medicine Grants [P20 GM103549] and [P30 GM118247]

Analysis of the quaternary structure of rhGPx3 mutant The purified rhGPx3 mutant was treated with loading buffer with or without -mercaptoethanol and boiling and the resulting product was separated by SDS-PAGE

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