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Experimental design After 20 days of growth, tissue culture plantlets were divided into five groups for experimental treatment: (1) T0 (untreated), (2) Cd48 (48 h of growth following the addition of 500 mol/L of CdCl 2 to MS solution), (3) GSH48 (48 h of growth following the addition of 500 mol/L of CdCl 2 and 400 mol/L of GSH to MS solution), (4) Cd96 (96 h of growth following the addition of 500 mol/L of CdCl 2 to MS solution), and (5) GSH96 (96 h of growth following the addition of 500 mol/L of CdCl 2 and 400 mol/L of GSH to MS solution)

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As previously mentioned, SLC7A11 high cancer cells are more vulnerable to glucose and glutamine starvation

10.1021/pr401179v J

Membranes were visualized on a ChemiDoc MP Imaging System (Bio-Rad) and protein bands quantified using Image Lab software (v6.1.0, Bio-Rad)