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Bioinformatics analysis Raw transcriptomic sequencing data were processed using the nf-core RNA-seq v3.12.0 pipeline from the nf-core collection of community-curated, reproducible workflows 59

In vitro testing on A549 and B16 cell lines identified 8-(pyrrolidin-1-ylmethyl)4-trifluoromethyl ESC as the optimal derivative, demonstrating 20-fold enhanced antiproliferative activity and threefold prolonged half-life in human liver S9, with structural analysis revealing that nitrogen-containing groups at C-8 substantially enhance both metabolic stability and anticancer potency (Pan et al

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Using low-quality peptides from unverified online sources, which may be mislabeled, contaminated, underdosed, or not intended for human use

Regulatory issues and concerns regarding the use of peptides in weight loss products also exist, as some peptides are not approved for use in humans or are being used off-label